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immpress hrp polymer kit  (Vector Laboratories)


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    Vector Laboratories immpress hrp polymer kit
    Immpress Hrp Polymer Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+on+mouse+(m%2Eo%2Em%2E+)+immpress+hrp+(peroxidase)+polymer+kit/Mouse+on+Mouse+(M%2EO%2EM%2E+)+ImmPRESS+HRP+(Peroxidase)+Polymer+Kit/pm42010768-68-0-6
    Average 95 stars, based on 164 article reviews
    immpress hrp polymer kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Saline:

    Article Title: Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC
    Article Snippet: Sections were incubated overnight in a humidified chamber at 4°C with primary antibodies (KRT6A: Proteintech Group, 17391-1-AP, 1:1000; KRT14: Thermo Fisher Scientific, MA5-11599, 1:1000; C15ORF48: Atlas Antibodies, HPA012943, 1:500; CTSV: Origene, TA590738, 1:500; and CLIP4: Atlas Antibodies, HPA043366, 1:500). .. Sections were washed with phosphate-buffered saline (PBS) and then incubated with either rabbit secondary antibody (Vector Laboratories, MP-7401) or mouse secondary antibody (Vector Laboratories, MP-2400) for 1 hour at RT. .. Sections were washed with PBS, incubated with ImmPACT DAB peroxidase [horseradish peroxidase (HRP)] substrate (Vector Laboratories, SK-4105), counterstained with hematoxylin QS (Vector Laboratories, H-3404), dehydrated through ethanol-to-xylene washes, and mounted using permanent mounting medium (VectraMount, H-5000).

    Incubation:

    Article Title: Regulation of mitochondrial ROS by C15ORF48 in a basal cell subpopulation contributes to chemotherapy resistance in TNBC
    Article Snippet: Sections were incubated overnight in a humidified chamber at 4°C with primary antibodies (KRT6A: Proteintech Group, 17391-1-AP, 1:1000; KRT14: Thermo Fisher Scientific, MA5-11599, 1:1000; C15ORF48: Atlas Antibodies, HPA012943, 1:500; CTSV: Origene, TA590738, 1:500; and CLIP4: Atlas Antibodies, HPA043366, 1:500). .. Sections were washed with phosphate-buffered saline (PBS) and then incubated with either rabbit secondary antibody (Vector Laboratories, MP-7401) or mouse secondary antibody (Vector Laboratories, MP-2400) for 1 hour at RT. .. Sections were washed with PBS, incubated with ImmPACT DAB peroxidase [horseradish peroxidase (HRP)] substrate (Vector Laboratories, SK-4105), counterstained with hematoxylin QS (Vector Laboratories, H-3404), dehydrated through ethanol-to-xylene washes, and mounted using permanent mounting medium (VectraMount, H-5000).

    Article Title: Impact of Cellular Senescence on LCN2 Expression in Salivary Gland Epithelial Cells and Oral Keratinocytes
    Article Snippet: Sections were treated with the following antibodies: Normal Rat IgG (1:500, #147‐09521, Fujifilm Wako), Normal Mouse IgG (1:5000, #140‐09511, Fujifilm Wako), Lipocalin‐2/NGAL (1:50, #ab70287, Abcam), or NGAL (1:100, # sc‐515876, Santa Cruz Biotechnology). .. After sections were incubated at 4°C overnight, they were washed with PBS and treated with the ImmPRESS HRP Goat Anti‐Rat IgG (Mouse Adsorbed) Polymer Kit (#MP‐7444, Vector Laboratories, Newark, CA, USA) or M.O.M ImmPRESS Peroxidase Polymer Kit (#MP‐2400, Vector Laboratories). .. The reaction was detected by diaminobenzidine staining (#MK210, TaKaRa).

    Article Title: Essential Role of Integrin-Linked Kinase in Keratinocyte Responses to Mechanical Strain
    Article Snippet: .. To detect α-smooth muscle actin, 7 μm paraffin-embedded epidermis sections were fixed in Zn buffer (0.1 M Tris-HCl, pH 7.8, 0.05% Ca(OAc) 2 , 0.5% Zn(OAc) 2 , and 0.5% ZnCl 2 ), and subjected to high-temperature antigen retrieval, with 10 mM sodium citrate (pH 6.0), followed by incubation with anti-α-SMA antibodies (Millipore Sigma, St. Louis, MO, USA, A2547) using Vectastain Elite ABC kits (PK-6100) and ImmPRESS reagent (Vector Laboratories, Burlingame, CA, USA, MP-2400). .. Immunoreactivity signals were visualized with ImmPACT NovaRED peroxidase substrate (Vector Laboratories, Newark, CA, USA, SK-4805), and tissues were counterstained with Methyl Green.

    Article Title: Essential Role of Integrin-Linked Kinase in Keratinocyte Responses to Mechanical Strain.
    Article Snippet: .. To detect α-smooth muscle actin, 7 μm paraffin-embedded epidermis sections were fixed in Zn buffer (0.1 M Tris-HCl, pH 7.8, 0.05% Ca(OAc)2, 0.5% Zn(OAc)2, and 0.5% ZnCl2), and subjected to high-temperature antigen retrieval, with 10 mM sodium citrate (pH 6.0), followed by incubation with anti-α-SMA antibodies (Millipore Sigma, St. Louis, MO, USA, A2547) using Vectastain Elite ABC kits (PK-6100) and ImmPRESS reagent (Vector Laboratories, Burlingame, CA, USA, MP-2400). .. Immunoreactivity signals were visualized with ImmPACT NovaRED peroxidase substrate (Vector Laboratories, Newark, CA, USA, SK-4805), and tissues were counterstained with Methyl Green.

    Article Title: Impact of Cellular Senescence on LCN2 Expression in Salivary Gland Epithelial Cells and Oral Keratinocytes.
    Article Snippet: Sections were treated with the following antibodies: Normal Rat IgG (1:500, #147- 09521, Fujifilm Wako), Normal Mouse IgG (1:5000, #140- 09511, Fujifilm Wako), Lipocalin- 2/NGAL (1:50, #ab70287, Abcam), or NGAL (1:100, # sc- 515876, Santa Cruz Biotechnology). .. After sections were incubated at 4°C overnight, they were washed with PBS and treated with the ImmPRESS HRP Goat Anti- Rat IgG (Mouse Adsorbed) Polymer Kit (#MP- 7444, Vector Laboratories, Newark, CA, USA) or M.O.M ImmPRESS Peroxidase Polymer Kit (#MP- 2400, Vector Laboratories). .. The reaction was detected by diaminobenzidine staining (#MK210, TaKaRa).

    Polymer:

    Article Title: Impact of Cellular Senescence on LCN2 Expression in Salivary Gland Epithelial Cells and Oral Keratinocytes
    Article Snippet: Sections were treated with the following antibodies: Normal Rat IgG (1:500, #147‐09521, Fujifilm Wako), Normal Mouse IgG (1:5000, #140‐09511, Fujifilm Wako), Lipocalin‐2/NGAL (1:50, #ab70287, Abcam), or NGAL (1:100, # sc‐515876, Santa Cruz Biotechnology). .. After sections were incubated at 4°C overnight, they were washed with PBS and treated with the ImmPRESS HRP Goat Anti‐Rat IgG (Mouse Adsorbed) Polymer Kit (#MP‐7444, Vector Laboratories, Newark, CA, USA) or M.O.M ImmPRESS Peroxidase Polymer Kit (#MP‐2400, Vector Laboratories). .. The reaction was detected by diaminobenzidine staining (#MK210, TaKaRa).

    Article Title: Transcriptome Analysis and Histological Validation Identified the HO-1-u-1 Cell Line as a Model for Perineural Invasion in Oral Squamous Cell Carcinoma.
    Article Snippet: Blocking was then performed using either Protein Block Serum- Free Readyto- use (X0909; Agilent, Santa Clara, CA, USA) or the M.O.M. .. ImmPRESS HRP Polymer Kit (MP- 2400; Vector Laboratories, Newark, CA, USA), in accordance with the respective manufacturer's protocols. .. The sections were incubated overnight at 4°C with a single primary antibody: a cocktail of anti- human cytokeratin antibodies (clones AE1/AE3, MNF116, and 34bE12; Agilent) to identify tumor cells or a monoclonal mouse anti- β3 tubulin 16000714, 0, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jop.70143 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [24/04/2026].

    Article Title: Spatial resolution of the metastatic osteosarcoma tumor microenvironment using immunolabeling across murine, canine and human lung
    Article Snippet: Immunohistochemical labeling was performed using Leica Biosystems’ BondRX autostainer and Bond Polymer Refine Detection Kit (LeicaBiosystems #DS9800) for each of the antibodies in all three species (murine, canine, and human) at the dilution and antigen retrieval conditions listed in Table . .. Labeling of murine tissues with anti-mouse antibodies was accomplished with the Mouse on Mouse ImmPRESS HRP Polymer Kit (#MP-2400, Vector Laboratories). ..

    Article Title: Impact of Cellular Senescence on LCN2 Expression in Salivary Gland Epithelial Cells and Oral Keratinocytes.
    Article Snippet: Sections were treated with the following antibodies: Normal Rat IgG (1:500, #147- 09521, Fujifilm Wako), Normal Mouse IgG (1:5000, #140- 09511, Fujifilm Wako), Lipocalin- 2/NGAL (1:50, #ab70287, Abcam), or NGAL (1:100, # sc- 515876, Santa Cruz Biotechnology). .. After sections were incubated at 4°C overnight, they were washed with PBS and treated with the ImmPRESS HRP Goat Anti- Rat IgG (Mouse Adsorbed) Polymer Kit (#MP- 7444, Vector Laboratories, Newark, CA, USA) or M.O.M ImmPRESS Peroxidase Polymer Kit (#MP- 2400, Vector Laboratories). .. The reaction was detected by diaminobenzidine staining (#MK210, TaKaRa).

    Labeling:

    Article Title: Spatial resolution of the metastatic osteosarcoma tumor microenvironment using immunolabeling across murine, canine and human lung
    Article Snippet: Immunohistochemical labeling was performed using Leica Biosystems’ BondRX autostainer and Bond Polymer Refine Detection Kit (LeicaBiosystems #DS9800) for each of the antibodies in all three species (murine, canine, and human) at the dilution and antigen retrieval conditions listed in Table . .. Labeling of murine tissues with anti-mouse antibodies was accomplished with the Mouse on Mouse ImmPRESS HRP Polymer Kit (#MP-2400, Vector Laboratories). ..

    Article Title: Pendrin is Upregulated by Corticosterone and Participates in its Pressor Response
    Article Snippet: .. Endogenous peroxidase activity was blocked by incubating the sections in 3% H 2 O 2 in distilled water for 45 min. For pendrin labeling, sections were blocked for 1 hour in M.O.M. blocker (MKB-2213, Vector Laboratories, Burlingame, CA) along with an unconjugated Fab fragment goat anti-mouse IgG (115–007-003, Jackson ImmunoResearch, West Grove, PA) and 15 min with 2.5% Normal Horse Serum (MP-2400, Vector Laboratories, Burlingame, CA). .. Sections were then incubated at 4°C overnight with primary antibody diluted in 1 x PBS, washed in PBS and incubated for 45 min in polymer-linked peroxidase-conjugated anti-mouse IgG diluted to 1:10 or 30 min in anti-rabbit IgG (Vector ImmPRESS, Vector Laboratories, Burlingame, CA), washed again with PBS, and exposed to diaminobenzidine (Vector DAB substrate kit) for 5 min.

    Activity Assay:

    Article Title: Pendrin is Upregulated by Corticosterone and Participates in its Pressor Response
    Article Snippet: .. Endogenous peroxidase activity was blocked by incubating the sections in 3% H 2 O 2 in distilled water for 45 min. For pendrin labeling, sections were blocked for 1 hour in M.O.M. blocker (MKB-2213, Vector Laboratories, Burlingame, CA) along with an unconjugated Fab fragment goat anti-mouse IgG (115–007-003, Jackson ImmunoResearch, West Grove, PA) and 15 min with 2.5% Normal Horse Serum (MP-2400, Vector Laboratories, Burlingame, CA). .. Sections were then incubated at 4°C overnight with primary antibody diluted in 1 x PBS, washed in PBS and incubated for 45 min in polymer-linked peroxidase-conjugated anti-mouse IgG diluted to 1:10 or 30 min in anti-rabbit IgG (Vector ImmPRESS, Vector Laboratories, Burlingame, CA), washed again with PBS, and exposed to diaminobenzidine (Vector DAB substrate kit) for 5 min.



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